The overview of DAP-Seq (DNA affinity purification sequencing)
Introduction In the realms of functional genomics and epigenetics, the elucidation of transcription factor binding sites (TFBS) has perennially stood as a focal point of research endeavors. Disruption of TFBS has been intricately linked to phenotypic diversity, encompassing pivotal adaptive traits in agriculture and various disease states, including cancer. The comprehensive mapping of individual TFs’…
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Principles, Workflow and Analysis of DAP-seq
Overview of DAP-seq The DNA Affinity Purification Sequencing (DAP-seq) technique is an innovative method aimed at exploring the interactions between transcription factors (TFs) and genomic DNA. By simulating the interaction between TFs and their DNA binding sites in vitro, this technology reveals key DNA sequences in gene regulatory networks. In contrast to traditional Chromatin Immunoprecipitation Sequencing (ChIP-seq), DAP-seq is…